Investigation of Thiol Disulphide Balance in Dogs with Canine Leishmaniasis

Özet

Previous studies have demonstrated that oxidative stress plays a significant role in the etiopathogenesis of diseases. In this study, it was aimed to elucidate the etiopathogenesis of Canine Leishmaniasis by comparing the levels of thiol/disulfide, known as oxidative stress markers, in affected dogs and clinically healthy controls. Specifically, the objective was to determine the levels of dynamic Thiol-Disulfide Homeostasis in serum, including native thiol, total thiol, and disulfide, as well as to calculate the ratios of disulfide/total thiol and disulfide/native thiol.

A total of 30 dogs aged between 1 and 9 years were included in the study. These dogs were divided into two groups: positive and negative, to form the experimental and control groups. Blood samples were collected from dogs suspected of having leishmaniasis in veterinary clinics (Caniv-4 Leishmania, Vet Expert, Vet Planet Ltd., Poland). Using a diagnostic kit screening for four infectious diseases, dogs that tested positive only for leishmaniasis and negative for the remaining three were included in the infected group, while dogs that tested negative for all were included in the control group. Serum samples were stored at −20°C and analyzed in the laboratory for thiol, disulfide, and ischemia-modified albumin (IMA) levels.

Thiol-disulfide and IMA levels of dogs diagnosed with leishmaniasis (n=20) and healthy dogs (n=10) were compared. Native thiol and total thiol levels in leishmaniasis-positive dogs were found to be similar to those of healthy controls. The findings suggest that oxidative stress may occur in dogs with Canine Leishmaniasis when thiol-disulfide homeostasis is used as a reference marker.