Investigation of the Seroprevalance of Canine Herpesvirus-1 in Dogs in Central Anatolian Region

Abstract

In this study; Canine herpesvirus (CHV-1) seroprevalence study, which causes infertility in breeding or pregnant dogs in various commercial dog breeding centers in the Central Anatolia region, was conducted to investigate the situation in the region.

In the study carried out in commercial dog breeding enterprises in various provinces and districts in the Central Anatolia Region (Ankara: Haymana, Gölbaşı, Çubuk, Etimesgut, Yenimahalle. Eskişehir: Seyit Gazi. Çankırı: Eldivan.); German Shepherd (n=13), Doberman (n=23), Rottweiller (n=31), Anatolian Shepherd Dog (Akbaş n=3, Malaklı n=9, Kangal n=29), Labrador (n=2), Mallinois  (n=2), Border Collie (n=1) and Bernese Mountain Dog (n=1) , a total of 114 breeds were included. One of the samples collected from each dog in two anticoagulant (EDTA) tubes was analyzed molecularly for PCR (Polymerase Chain Reaction) process, and the other serological evaluation was made with the commercial CHV-1 ELISA kit and these results were compared. Canine Herpes Virus-1 antibody levels were found to be above 10 ng/mL in all establishments where the study was carried out. It was determined that there were many pathological findings in the reproductive history of antibody positive cases.

As a result of this study, it was thought that the dogs to be used in breeding, whether female or male, should be subjected to series of gynecological examinations and especially animals free from Canine Herpes Virus-1 should be used in production.

Keywords: Canine Herpesvirus, Infertility, Canine, Seroprevalence, PCR, ELISA